6Z01
DNA Topoisomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979030 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.200, 96.590, 94.560 |
| Unit cell angles | 90.00, 113.33, 90.00 |
Refinement procedure
| Resolution | 35.440 - 1.900 |
| R-factor | 0.1816 |
| Rwork | 0.180 |
| R-free | 0.21670 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.440 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 98987 | 9850 |
| <I/σ(I)> | 16.17 | |
| Completeness [%] | 99.8 | 99.96 |
| Redundancy | 9.85 | |
| CC(1/2) | 0.983 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG 6000 1 M LiCl 100mM Sodium Acetate |
