6YZZ

Arabidopsis thaliana Naa50 in complex with AcCoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0781
Spacegroup name	I 2 3
Unit cell lengths	100.164, 100.164, 100.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.890 - 1.790
R-factor	0.1713
Rwork	0.170
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ob0
RMSD bond length	0.007
RMSD bond angle	0.921
Data reduction software	XDS (VERSION Nov 11, 2017 BUILT=20171111)
Data scaling software	Aimless (7.0.073)
Phasing software	PHASER (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.890	1.820
High resolution limit [Å]	1.790	1.790
Rpim	0.019	0.752
Number of reflections	16046	914
<I/σ(I)>	21.2	1
Completeness [%]	100.0	100
Redundancy	7.5	7.6
CC(1/2)	1.000	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	34 mg mL-1 protein (20 mM HEPES pH 8.0, 250 mM NaCl) mixed with AcCoA (5.4 mM); drops (600 nL): 1:1 mix of (20 mM HEPES pH 8.0, 250 mM NaCl) and (0.9 M sodium citrate, 0.1 M sodium cacodylate pH 6.5). Growth after 19 h, 20 % glycerol (v/v) as cryo protection