Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 154.598, 154.598, 229.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 128.187 - 3.300 |
| Rwork | 0.238 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yla |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.390 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.187 | 3.350 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rpim | 0.094 | 4.094 |
| Number of reflections | 40954 | 1538 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 98.0 | |
| Redundancy | 78 | |
| CC(1/2) | 0.994 | 0.369 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | The best crystals were grown in condition containing 0.1 M sodium citrate tribasic dihydrate, pH 5.0, 10% (w/v) Polyethylene glycol 6000. |
