6YZ2
Full length Open-form Sodium Channel NavMs F208L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9797 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 108.940, 108.940, 209.367 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 104.680 - 2.200 |
R-factor | 0.2438 |
Rwork | 0.243 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hvx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.820 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.680 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 32388 | 2771 |
<I/σ(I)> | 17.4 | 2.2 |
Completeness [%] | 100.0 | |
Redundancy | 21.2 | |
CC(1/2) | 0.999 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M lithium sulphate, 0.1M HEPES, pH 7, and 40% v/v PEG200. |