6YYM
Structure of S. pombe Mei2 RRM3 domain bound to RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 75.270, 81.480, 81.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.690 - 2.630 |
| R-factor | 0.227 |
| Rwork | 0.223 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yyl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.790 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 7585 | 1118 |
| <I/σ(I)> | 5.9 | 1.06 |
| Completeness [%] | 98.0 | 92.5 |
| Redundancy | 5.5 | |
| CC(1/2) | 0.988 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 278 | 0.2M NaCl, 0.1M Na/K phosphate pH6.5, 25% w/v PEG 1000 |
