6YYM

Structure of S. pombe Mei2 RRM3 domain bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97857
Spacegroup name	I 2 2 2
Unit cell lengths	75.270, 81.480, 81.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.690 - 2.630
R-factor	0.227
Rwork	0.223
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yyl
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.790
High resolution limit [Å]	2.630	2.630
Number of reflections	7585	1118
<I/σ(I)>	5.9	1.06
Completeness [%]	98.0	92.5
Redundancy	5.5
CC(1/2)	0.988	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	278	0.2M NaCl, 0.1M Na/K phosphate pH6.5, 25% w/v PEG 1000