6YY1
Arabidopsis aspartate transcarbamoylase in apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.690, 109.570, 208.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 92.850 - 3.060 |
R-factor | 0.1929 |
Rwork | 0.190 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ypo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.011 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.850 | 3.180 |
High resolution limit [Å] | 3.060 | 3.060 |
Rmerge | 0.140 | 0.830 |
Number of reflections | 45073 | 4435 |
<I/σ(I)> | 11.16 | 2.33 |
Completeness [%] | 99.3 | 99.57 |
Redundancy | 5.2 | |
CC(1/2) | 0.994 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Protein at 5 mg/ml in buffer 20 mM Tris pH 7.0, 0.1 M NaCl, 2% glycerol, 0.2 mM tris(2-carboxyethyl) phosphine (TCEP). Crystallization solution was 18-22% PEG 3350 and 150-200 mM potassium acetate. Crystals were cryo-protected by soaking in a solution containing the mother liquor supplemented with 20% glycerol |