6YXW
Affimer K3 - KRAS protein complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-27 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9660 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.126, 39.490, 113.212 |
Unit cell angles | 90.00, 106.91, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.160 | 2.110 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.089 | 0.393 |
Rpim | 0.063 | 0.465 |
Number of reflections | 38740 | 2830 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 2.9 | 3 |
CC(1/2) | 0.978 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 2M ammonium sulfate, 0.2M potassium sodium tartrate, 0.1M tri-sodium citrate |