6YXP
Higher resolution structure of the N-terminal module of the human SWI/SNF-subunit BAF155/SMARCC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.939273 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.900, 138.322, 56.510 |
Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
Resolution | 42.066 - 1.600 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.19780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6yxo |
RMSD bond length | 0.006 |
RMSD bond angle | 0.800 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.490 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.053 | 0.611 |
Rmeas | 0.057 | 0.661 |
Rpim | 0.022 | 0.248 |
Number of reflections | 82320 | 11930 |
<I/σ(I)> | 15.1 | 2.7 |
Completeness [%] | 99.1 | 98.2 |
Redundancy | 6.8 | 6.9 |
CC(1/2) | 0.999 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 290 | 0.2 M Magnesium formate (pH 5.9), 20% PEG 3350. Protein at 16 mg/ml. |