6YXO

Structure of the N-terminal module of the human SWI/SNF-subunit BAF155/SMARCC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	DIAMOND
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.975456
Spacegroup name	P 1 21 1
Unit cell lengths	43.810, 136.815, 56.042
Unit cell angles	90.00, 110.52, 90.00

Refinement procedure

Resolution	49.005 - 2.000
R-factor	0.1993
Rwork	0.197
R-free	0.23820
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.628
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.010	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.432
Rmeas	0.086	0.533
Rpim	0.050	0.306
Number of reflections	41433	5994
<I/σ(I)>	10.9	2.9
Completeness [%]	99.5	99.3
Redundancy	5.4	5.6
CC(1/2)	0.997	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	290	0.2 M Magnesium formate (pH 5.9), 20% PEG 3350. Protein at 16 mg/ml.