6YXO
Structure of the N-terminal module of the human SWI/SNF-subunit BAF155/SMARCC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.975456 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.810, 136.815, 56.042 |
| Unit cell angles | 90.00, 110.52, 90.00 |
Refinement procedure
| Resolution | 49.005 - 2.000 |
| R-factor | 0.1993 |
| Rwork | 0.197 |
| R-free | 0.23820 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.628 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.432 |
| Rmeas | 0.086 | 0.533 |
| Rpim | 0.050 | 0.306 |
| Number of reflections | 41433 | 5994 |
| <I/σ(I)> | 10.9 | 2.9 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 5.4 | 5.6 |
| CC(1/2) | 0.997 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 290 | 0.2 M Magnesium formate (pH 5.9), 20% PEG 3350. Protein at 16 mg/ml. |
