6YXE

Structure of the Trim69 RING domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.2524
Spacegroup name	H 3 2
Unit cell lengths	109.965, 109.965, 90.135
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.460 - 2.100
R-factor	0.1999
Rwork	0.198
R-free	0.23950
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.570
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	SHELXDE
Refinement software	REFMAC (5.0.32)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.460	42.100	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.111	0.042	2.145
Rmeas	0.113	0.044	2.219
Rpim	0.024	0.010	0.558
Total number of observations		3484	15246
Number of reflections	12387	182	999
<I/σ(I)>	27.4	107.1	1.5
Completeness [%]	100.0	99.2	100
Redundancy	21.5	19.1	15.3
CC(1/2)	1.000	1.000	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	1.26 M NH4SO4, 0.2 M LiSO4, and 0.1 M Tris pH 8.5