6YXD
Room temperature structure of human adiponectin receptor 2 (ADIPOR2) at 2.9 A resolution determined by Serial Crystallography (SSX) using CrystalDirect
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-07-01 |
Detector | DECTRIS EIGER2 X 4M |
Wavelength(s) | 0.988 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 75.460, 100.760, 114.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.25310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lwy |
RMSD bond length | 0.006 |
RMSD bond angle | 0.820 |
Data reduction software | CrystFEL (0.8.0) |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 15292 | 1971 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 100.0 | |
Redundancy | 130.57 | |
CC(1/2) | 0.980 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | CrystalDirect-2 plates (MiTeGen) in 30 or 50 nL bolus overlaid with 600 nl precipitant solution. 42.3% PEG 400, 110 mM potassium citrate, 100 mM HEPES pH 7.0 for ADIPOR2. |