6YX9

Cryogenic human adiponectin receptor 2 (ADIPOR2) at 2.4 A resolution determined by Serial Crystallography (SSX) using CrystalDirect

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 2 21
Unit cell lengths	74.720, 100.580, 109.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.000 - 2.404
R-factor	0.209
Rwork	0.207
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lwy
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	30556	2326
<I/σ(I)>	8.3	1.02
Completeness [%]	100.0
Redundancy	37.12
CC(1/2)	0.991	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	42.3% PEG 400, 110 mM potassium citrate, 100 mM HEPES pH 7.0. Crystallisation experiments were carried out at the HTX facility of EMBL-Grenoble. Automated crystal harvesting under cryogenic condition was done by CrystalDirect technology