6YX8
The structure of allophycocyanin from cyanobacterium Nostoc sp. WR13, the C2221 crystal form.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 104.676, 177.340, 122.416 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.144 - 1.831 |
Rwork | 0.160 |
R-free | 0.19830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6yx7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.547 |
Data reduction software | XDS (Jan 26, 2018 (20180409)) |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.144 | 1.941 |
High resolution limit [Å] | 1.831 | 1.831 |
Rmerge | 0.126 | 1.278 |
Rmeas | 0.137 | 1.278 |
Rpim | 0.053 | 0.524 |
Number of reflections | 87249 | 4360 |
<I/σ(I)> | 9.3 | 1.5 |
Completeness [%] | 95.7 | 58.3 |
Redundancy | 6.6 | |
CC(1/2) | 0.996 | 0.599 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Morpheus screen condition E12: 37.5% v/v Precipitant mix 4: 25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350; 0.1M Buffer system 3: 1.0M Tris (base); bicine, pH 8.5 0.12M Additives: 0.3M ethylene glycols |