6YX7
The high resolution structure of allophycocyanin from cyanobacterium Nostoc sp. WR13, the P21212 crystal form.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97932 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 123.695, 177.726, 106.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.372 - 1.419 |
Rwork | 0.126 |
R-free | 0.17510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b33 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.517 |
Data reduction software | XDS (June 26, 2018 (20180409)) |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.372 | 1.503 |
High resolution limit [Å] | 1.419 | 1.419 |
Rmerge | 0.077 | 1.220 |
Rmeas | 0.082 | 1.313 |
Rpim | 0.027 | 0.478 |
Number of reflections | 383047 | 19080 |
<I/σ(I)> | 13.8 | 1.7 |
Completeness [%] | 96.1 | 61.3 |
Redundancy | 8.7 | 7.2 |
CC(1/2) | 0.998 | 0.623 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Morpheus screen condition H12: Precipitant mix 4: 25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350; Buffer system 3: 1.0M Tris (base); bicine, pH 8.5 Additives: amino acids: 0.2M Gly, DL-Ala, DL-Ser, DL-Glu, DL-Lys |