6YX6
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148K variant (no morpholine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9737 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.772, 110.839, 39.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 1.500 |
R-factor | 0.1754 |
Rwork | 0.174 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6yx4 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.329 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.993 | 1.539 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 35712 | 1786 |
<I/σ(I)> | 14.8 | 2.9 |
Completeness [%] | 86.6 | 59.9 |
Redundancy | 5.6 | 5.6 |
CC(1/2) | 0.999 | 0.881 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.5M Ammonium Sulfate, 0.1M tri-Sodium citrate, pH 6.5, 1M Lithium sulfate |