6YWR
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.967, 54.035, 39.229 |
| Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
| Resolution | 54.870 - 1.500 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rhf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.323 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.870 | 54.870 | 1.530 |
| High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
| Rmerge | 0.071 | 0.063 | 0.490 |
| Rmeas | 0.085 | 0.077 | 0.586 |
| Rpim | 0.046 | 0.042 | 0.318 |
| Total number of observations | 114941 | 694 | 5784 |
| Number of reflections | 35488 | 237 | 1785 |
| <I/σ(I)> | 9.5 | 20.1 | 2.3 |
| Completeness [%] | 96.4 | 97.1 | 99.2 |
| Redundancy | 3.2 | 2.9 | 3.2 |
| CC(1/2) | 0.995 | 0.992 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.5 M Lithium sulfate monohydrate, 15% w/v PEG 8000 |
