6YWC
De novo designed protein 4E1H_95 in complex with 101F antibody
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 181.146, 65.417, 128.525 |
| 格子定数 [度] | 90.00, 119.35, 90.00 |
精密化法
| 分解能 | 49.450 - 2.850 |
| R因子 | 0.2144 |
| Rwork | 0.212 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o41 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.912 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 49.500 | 2.840 |
| 分解能 [Å] (高) | 2.670 | 2.670 |
| 独立反射数 | 71869 | 11512 |
| <I/σ(I)> | 7.7 | 1.1 |
| 完全性 [%] | 99.5 | 98.5 |
| 冗長性 | 3.6 | |
| CC(1/2) | 0.987 | 0.398 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium citrate pH 5.8, 0.1 M ammonium sulfate, 16% w/v PEG 4000, 20% v/v glycerol |
