6YWB
Human wtSTING in complex with 3',3'-c-[2'FdAMP-2'FdAM(PS)]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-09 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 110.906, 110.906, 35.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.070 - 2.503 |
R-factor | 0.2117 |
Rwork | 0.209 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ksy |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.070 | 2.593 |
High resolution limit [Å] | 2.503 | 2.503 |
Rmerge | 0.162 | 1.031 |
Rpim | 0.060 | 0.371 |
Number of reflections | 8087 | 769 |
<I/σ(I)> | 10.69 | |
Completeness [%] | 99.5 | |
Redundancy | 8.2 | |
CC(1/2) | 0.997 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.0 M Sodium/Potassium tartrate, 0.2 M Lithium sulfate, 0.1 M Tris pH 7.0 |