6YWA

Human REF STING in complex with 3',3'-c-[2'FdAMP-2'FdAM(PS)]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 2 21 21
Unit cell lengths	35.959, 94.244, 116.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.959 - 2.311
R-factor	0.1963
Rwork	0.194
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ksy
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.960	2.394
High resolution limit [Å]	2.311	2.311
Rmerge	0.116	0.518
Rmeas	0.131	0.585
Rpim	0.059	0.268
Number of reflections	18010	1751
<I/σ(I)>	10.9
Completeness [%]	99.3
Redundancy	4.8
CC(1/2)	0.996	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Citric acid pH 5.0, 20% PEG 6000