6YWA
Human REF STING in complex with 3',3'-c-[2'FdAMP-2'FdAM(PS)]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-10 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 35.959, 94.244, 116.826 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.959 - 2.311 |
R-factor | 0.1963 |
Rwork | 0.194 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ksy |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.960 | 2.394 |
High resolution limit [Å] | 2.311 | 2.311 |
Rmerge | 0.116 | 0.518 |
Rmeas | 0.131 | 0.585 |
Rpim | 0.059 | 0.268 |
Number of reflections | 18010 | 1751 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 99.3 | |
Redundancy | 4.8 | |
CC(1/2) | 0.996 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Citric acid pH 5.0, 20% PEG 6000 |