6YVP
Human histidine triad nucleotide-binding protein 2 (hHINT2) complexed with dGMP and refined to 2.77 A
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-07 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.649, 36.531, 86.887 |
Unit cell angles | 90.00, 103.03, 90.00 |
Refinement procedure
Resolution | 42.320 - 2.770 |
Rwork | 0.208 |
R-free | 0.30770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6YQD:B |
RMSD bond length | 0.005 |
RMSD bond angle | 1.491 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP (11.7.02) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.320 | 2.920 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.198 | 0.668 |
Rmeas | 0.266 | 0.894 |
Rpim | 0.176 | 0.589 |
Number of reflections | 5698 | 814 |
<I/σ(I)> | 5.5 | 2 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 3.8 | 3.9 |
CC(1/2) | 0.985 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281 | 15 % (w/v) PEG6000, 5 % (v/v) glycerol |