6YVG
Crystal structure of MesI (Lpg2505) from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.960, 56.910, 70.920 |
Unit cell angles | 90.00, 99.61, 90.00 |
Refinement procedure
Resolution | 44.140 - 2.200 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.23040 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.140 | 44.140 | 2.300 |
High resolution limit [Å] | 2.200 | 20.000 | 2.200 |
Rmerge | 0.069 | 0.045 | 0.960 |
Rmeas | 0.082 | 0.055 | 1.132 |
Total number of observations | 107228 | ||
Number of reflections | 15667 | 35 | 3770 |
<I/σ(I)> | 9.74 | 24.09 | 1.25 |
Completeness [%] | 99.6 | 85.4 | 98.3 |
Redundancy | 3.529 | 2.686 | 3.523 |
CC(1/2) | 0.998 | 0.994 | 0.622 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM ammonium iodide, 20 % w/v PEG3350 |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM ammonium iodide, 20 % w/v PEG3350 |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM ammonium iodide, 20 % w/v PEG3350 |