6YV7

Mannosyltransferase PcManGT from Pyrobaculum calidifontis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.06884
Spacegroup name	P 21 21 21
Unit cell lengths	51.449, 107.608, 163.572
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.079 - 2.700
R-factor	0.2505
Rwork	0.247
R-free	0.29020
Structure solution method	SIRAS
RMSD bond length	0.014
RMSD bond angle	1.878
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	48112	4916
<I/σ(I)>	12.2	2.5
Completeness [%]	99.9	99.8
Redundancy	7
CC(1/2)	0.997	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 mM HEPES (pH 7.5), 20% PEG 4000, 10% isopropanol, 10 mM MnCl2 and 2% 1,2,3-heptanetriol