6YUN

1.45 Angstrom Resolution Crystal Structure of C-terminal Dimerization Domain of Nucleocapsid Phosphoprotein from SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	43.430, 46.856, 131.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.150 - 1.440
R-factor	0.1499
Rwork	0.148
R-free	0.18880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cjr
RMSD bond length	0.011
RMSD bond angle	1.103
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_3758: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.860	1.520
High resolution limit [Å]	1.440	1.440
Rmerge	0.034
Number of reflections	411193	44155
<I/σ(I)>	18.4
Completeness [%]	98.4
Redundancy	8.5
CC(1/2)	0.998	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	277	31% PEG 4K 0.2 M Lithium sulfate 50 mM Tris pH 7.8