Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999998 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.649, 128.649, 125.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.480 - 2.400 |
| Rwork | 0.217 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pe2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.459 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.480 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.027 | 0.426 |
| Number of reflections | 41786 | 4317 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 14.9 | |
| CC(1/2) | 0.999 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M ammonia sulphate, 0.1 MES pH 6.5, 31-35% (v/v) polyethylene glycol PEG 5000 MME 10 mg / mL protein |
