6YUD
Structure of Csx3/Crn3 from Archaeoglobus fulgidus in complex with cyclic tetra-adenylate (cA4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-29 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.969, 60.364, 107.085 |
| Unit cell angles | 90.00, 116.47, 90.00 |
Refinement procedure
| Resolution | 53.560 - 1.840 |
| R-factor | 0.1947 |
| Rwork | 0.192 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wzi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.623 |
| Data reduction software | xia2 (0.5.900-ga3de5862-dials-1.14) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.560 | 53.560 | 1.890 |
| High resolution limit [Å] | 1.840 | 8.230 | 1.840 |
| Rmerge | 0.044 | 0.029 | 0.724 |
| Rmeas | 0.053 | 0.035 | 0.913 |
| Rpim | 0.029 | 0.019 | 0.547 |
| Total number of observations | 302768 | 3937 | 12833 |
| Number of reflections | 93596 | 1160 | 5287 |
| <I/σ(I)> | 11.3 | 46 | 1.1 |
| Completeness [%] | 97.1 | 99.6 | 74.5 |
| Redundancy | 3.2 | 3.4 | 2.4 |
| CC(1/2) | 0.995 | 0.976 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 100mM Hepes 25% (v/v) Jeffamine M-600 |
