6YUC
Crystal structure of Uba4-Urm1 from Chaetomium thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 197.735, 197.735, 99.566 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.870 - 3.150 |
R-factor | 0.2131 |
Rwork | 0.210 |
R-free | 0.26310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292108208 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.525 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.870 | 3.263 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.062 | 5.447 |
Rmeas | 0.066 | 4.720 |
Rpim | 0.021 | 1.910 |
Number of reflections | 13003 | 1279 |
<I/σ(I)> | 18.85 | |
Completeness [%] | 99.8 | |
Redundancy | 9.9 | |
CC(1/2) | 1.000 | 0.297 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5 and 20 % (w/v) PEG 3350 |