6YU2
Crystal structure of MhsT in complex with L-isoleucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00001 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.950, 97.290, 110.940 |
Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
Resolution | 43.698 - 3.100 |
R-factor | 0.2783 |
Rwork | 0.277 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4us3 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.700 | 3.310 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.195 | |
Number of reflections | 13856 | 2557 |
<I/σ(I)> | 3.6 | |
Completeness [%] | 81.6 | |
Redundancy | 2.7 | |
CC(1/2) | 0.989 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | Crystallized using HiLiDe with DOPC as added lipid. Crystals were obtained in 14-24% PEG400, 0.3-0.5M NaCl, 0.1 M Tris-HCl or HEPES-NaOH pH 7.0, 5% Trimethylamine N-oxide (TMANO), 5% or 10% glycerol. |