6YTN
Magnesium chelatase H subunit (ChlH) E660W variant from Synechocystis sp.PCC6803
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97630 |
Spacegroup name | H 3 |
Unit cell lengths | 317.550, 317.550, 104.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 79.387 - 2.700 |
Rwork | 0.192 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ysg |
RMSD bond length | 0.004 |
RMSD bond angle | 1.294 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.390 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.059 | 0.676 |
Number of reflections | 107741 | 7995 |
<I/σ(I)> | 10.2 | 1.3 |
Completeness [%] | 99.9 | |
Redundancy | 5.8 | |
CC(1/2) | 0.997 | 0.490 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.8-1.2 M sodium citrate and 2 mM dithiothreitol |