6YTJ
Magnesium chelatase H subunit (ChlH) E625K variant from Synechocystis sp.PCC6803
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | H 3 |
| Unit cell lengths | 320.422, 320.422, 104.876 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.105 - 2.790 |
| Rwork | 0.209 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ysg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.381 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.110 | 2.860 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rpim | 0.074 | 0.659 |
| Number of reflections | 99929 | 7451 |
| <I/σ(I)> | 6.4 | 1.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.993 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.8-1.2 M sodium citrate and 2 mM dithiothreitol |
