6YT3
Structure of the MoStoNano fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 165.697, 165.697, 352.162 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.890 - 2.850 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6t |
RMSD bond length | 0.009 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.890 | 2.980 |
High resolution limit [Å] | 2.850 | 2.850 |
Rpim | 0.071 | 0.351 |
Number of reflections | 33352 | 1670 |
<I/σ(I)> | 8.2 | 2.3 |
Completeness [%] | 99.9 | |
Redundancy | 10.6 | |
CC(1/2) | 0.998 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M tri-Sodium citrate pH 5.6, 10% PEG 4000, 10% Isopropanol |