6YT3
Structure of the MoStoNano fusion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 165.697, 165.697, 352.162 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.890 - 2.850 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f6t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.890 | 2.980 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rpim | 0.071 | 0.351 |
| Number of reflections | 33352 | 1670 |
| <I/σ(I)> | 8.2 | 2.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.998 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M tri-Sodium citrate pH 5.6, 10% PEG 4000, 10% Isopropanol |
