6YS9

T_926 truncate of ChlH from Thermosynechococcus elongatus at 1.64 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97633
Spacegroup name	P 1 21 1
Unit cell lengths	76.480, 140.330, 78.010
Unit cell angles	90.00, 108.66, 90.00

Refinement procedure

Resolution	64.380 - 1.640
R-factor	0.203
Rwork	0.201
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet poly-Ala model
RMSD bond length	0.008
RMSD bond angle	1.545
Data reduction software	XDS
Data scaling software	Aimless (0.5.1)
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.380	1.680
High resolution limit [Å]	1.640	1.640
Rmerge	0.073	0.544
Rmeas	0.093	0.704
Rpim	0.057	0.442
Number of reflections	172739	10643
<I/σ(I)>	7.4
Completeness [%]	91.2	76.3
Redundancy	2.3	2.2
CC(1/2)	0.994	0.487

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	290	20-22% PEG 6000, 100 mM Tris pH 8, 200 mM MgCl2