6YRY

tRNA-Guanine Transglycosylase (TGT) H333A mutant crystallised at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-04-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	90.315, 65.147, 70.891
Unit cell angles	90.00, 96.48, 90.00

Refinement procedure

Resolution	44.870 - 1.820
R-factor	0.1768
Rwork	0.175
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pud
RMSD bond length	0.006
RMSD bond angle	0.779
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.870	1.930
High resolution limit [Å]	1.820	1.820
Number of reflections	35506	5627
<I/σ(I)>	9.43
Completeness [%]	97.1
Redundancy	2.9
CC(1/2)	0.997	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	288	100 mM MES pH 5.5, 0.5 mM DTT, 10% (v/v) DMSO, 13% (m/v) PEG8000, streak seeding