6YRT
Transaldolase variant T30D from T. acidophilum in complex with D-fructose 6-phosphate Schiff-base intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 149.042, 172.522, 100.402 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 112.784 - 1.650 |
R-factor | 0.1689 |
Rwork | 0.168 |
R-free | 0.18660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s0c |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 112.784 | 100.000 | 1.750 |
High resolution limit [Å] | 1.650 | 50.000 | 1.650 |
Rmerge | 0.039 | 0.039 | 1.103 |
Rmeas | 0.041 | 0.048 | 1.145 |
Total number of observations | 2087960 | ||
Number of reflections | 153963 | 5 | 24717 |
<I/σ(I)> | 29.59 | 33.85 | 2.91 |
Completeness [%] | 99.6 | 62.5 | 99.8 |
Redundancy | 13.561 | 2.6 | 13.761 |
CC(1/2) | 0.999 | 0.996 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 188 mM ammonium acetate (pH 4.4), 10% (w/v) PEG 6000, and 25% (v/v) glycerol |