6YRO
Streptococcus suis SadP mutant - N285D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.132, 80.799, 130.740 |
Unit cell angles | 90.00, 113.13, 90.00 |
Refinement procedure
Resolution | 45.680 - 2.050 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.22180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bob |
RMSD bond length | 0.008 |
RMSD bond angle | 0.932 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.300 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmeas | 0.163 | 0.717 |
Number of reflections | 71993 | 3213 |
<I/σ(I)> | 6.2 | 1.5 |
Completeness [%] | 94.9 | 69 |
Redundancy | 3.7 | 2.9 |
CC(1/2) | 0.989 | 0.591 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.3-1.5 M sodium citrate tribasic, 0.1 M sodium cacodylate |