6YRN
Structure of the Chlamydomonas reinhardtii SAS-6 coiled-coil domain, P2 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.510, 39.532, 158.871 |
| Unit cell angles | 90.00, 101.34, 90.00 |
Refinement procedure
| Resolution | 81.530 - 2.430 |
| R-factor | 0.283 |
| Rwork | 0.282 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yrl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.820 | 2.470 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.154 | 0.855 |
| Rmeas | 0.185 | 1.065 |
| Rpim | 0.101 | 0.601 |
| Number of reflections | 40623 | 2015 |
| <I/σ(I)> | 3.7 | 1.1 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 3.2 | 2.9 |
| CC(1/2) | 0.995 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M carboxylic acid mixture, 0.1 M imidazole / MES buffer 20% v/v ethylene glycol 10% w/v PEG 8000 |
