6YQQ

ForT-PRPP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.916
Spacegroup name	P 41 21 2
Unit cell lengths	81.084, 81.084, 110.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.470 - 2.500
R-factor	0.2273
Rwork	0.225
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	an undeposited protein
RMSD bond length	0.005
RMSD bond angle	1.129
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.980	2.120
High resolution limit [Å]	2.080	2.080
Number of reflections	22946	1102
<I/σ(I)>	13.2
Completeness [%]	100.0
Redundancy	12.5
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	potassium dihydrogen phosphate, glycerol, PEG 8000, Bis-tris Propane