6YQ5
Hybrid structure of the SPP1 tail tube by solid-state NMR and cryo EM - NMR Ensemble
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
1 | Bruker | AVANCE | 900 |
Experiment
experiment id | conditions id | solution id | Experiment type |
1 | 1 | 1 | 4D HNhhNH |
10 | 1 | 2 | 3D HNhH |
11 | 1 | 3 | 3D HNhH |
12 | 1 | 4 | 3D HNhH |
13 | 1 | 5 | 3D HNhH |
14 | 1 | 9 | 3D HNhH |
15 | 1 | 10 | 3D HNhH |
16 | 1 | 8 | 3D HNhH |
17 | 1 | 7 | 3D HNhH |
18 | 1 | 6 | 3D HNhH |
2 | 1 | 2 | 3D HChH |
3 | 1 | 6 | 3D HChH |
4 | 1 | 2 | 2D hCH |
5 | 1 | 4 | 2D hCH |
6 | 1 | 6 | 2D hCH |
7 | 1 | 8 | 2D hCH |
8 | 1 | 10 | 2D hCH |
9 | 1 | 11 | 2D hCH |
NMR Sample
conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
1 | 7.4 | 1 | 291 |
Conformers
Conformers Calculated Total Number | 500 |
Conformers Submitted Total Number | 10 |
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TECNAI ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 4 |
Software | RELION,CTFFIND |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |