6YP9
Rabbit muscle actin in complex with ADF-H and ATP-ATTO-488
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.552, 69.017, 147.948 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.550 - 2.564 |
R-factor | 0.1767 |
Rwork | 0.174 |
R-free | 0.22990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3daw |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | XDS (BUILT=20200131 (Jan 31, 2020)) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.5.6) |
Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.546 | 2.608 |
High resolution limit [Å] | 2.564 | 2.564 |
Rmerge | 0.146 | 0.885 |
Rmeas | 0.161 | 0.969 |
Rpim | 0.065 | 0.390 |
Number of reflections | 17247 | 822 |
<I/σ(I)> | 10.3 | 2.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6 | 6.1 |
CC(1/2) | 0.996 | 0.701 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M sodium cacodylate (pH 6.0) and 15% (w/v) PEG 4000 |