6YP6
Structural annotation of the conserved carbohydrate esterase vb_24B_21 from Shiga toxin-encoding bacteriophage phi24B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.7514 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.522, 54.824, 72.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.490 - 0.970 |
R-factor | 0.1512 |
Rwork | 0.150 |
R-free | 0.17190 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.983 |
Data reduction software | XDS (VERSION Mar 15, 2019) |
Data scaling software | XDS (VERSION Mar 15, 2019) |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 0.980 |
High resolution limit [Å] | 0.970 | 0.970 |
Number of reflections | 128671 | 3878 |
<I/σ(I)> | 14.64 | 1 |
Completeness [%] | 99.5 | |
Redundancy | 7.1 | |
CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.9 M LiSO4, 10% [v/v] glycerol, 0.1 M Hepes pH 7.0 |