6YON
Crystal structure of Endoglucanase S127C/A165C from Penicillium verruculosum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2019-09-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978565037251 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.500, 85.690, 91.150 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 49.340 - 2.600 |
R-factor | 0.23923 |
Rwork | 0.236 |
R-free | 0.29197 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i6s |
RMSD bond length | 0.007 |
RMSD bond angle | 1.608 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.340 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 48284 | 3354 |
<I/σ(I)> | 5.15 | |
Completeness [%] | 99.2 | |
Redundancy | 4.08 | |
CC(1/2) | 0.994 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 285 | PEG 6000,Bis-Tris-Propane, MPD, tert-butanol |