6YOD
Structure of Lysozyme from SiN IMISX setup collected by rotation serial crystallography on crystals prelocated by 2D X-ray phase-contrast imaging
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.990, 78.990, 38.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.495 - 1.601 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d5f |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_3494) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.495 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.210 | 12.170 |
| Number of reflections | 16419 | 1147 |
| <I/σ(I)> | 13.58 | 0.86 |
| Completeness [%] | 99.9 | |
| Redundancy | 40.98 | 36.73 |
| CC(1/2) | 0.990 | 0.260 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.5 | 293 | 0.5-1 M NaBr, 50-100 mM CH3COONa, pH 4.5, and 15-30 %(v/v) PEG400 |
