6YOC

Structure of Lysozyme from COC IMISX setup collected by still serial crystallography on crystals prelocated by 2D X-ray phase-contrast imaging

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	79.170, 79.170, 38.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.980 - 1.860
R-factor	0.2208
Rwork	0.220
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d5f
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.980	1.890
High resolution limit [Å]	1.850	1.850
Rmeas	0.240	0.950
Number of reflections	10598	306
<I/σ(I)>	3.59	1.32
Completeness [%]	100.0
Redundancy	31.1	16.7
CC(1/2)	0.700	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.5	293	0.5-1 M NaBr, 50-100 mM CH3COONa, pH 4.5, and 15-30 %(v/v) PEG400