6YOA

Lig v 1 structure and the inflammatory response to the Ole e 1 protein family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	90
Detector technology	PIXEL
Collection date	2019-04-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	P 31 2 1
Unit cell lengths	76.484, 76.484, 113.498
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.095 - 2.830
R-factor	0.2645
Rwork	0.262
R-free	0.31930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z8w
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.100	2.830
High resolution limit [Å]	2.830	2.830
Rmerge	0.042	0.324
Number of reflections	9565	937
<I/σ(I)>	6.66
Completeness [%]	99.7	99.68
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290.15	0.1 M Tris pH 8.5 1 mM NiCl2 25% PEG MME 2000