6YO9

Product bound structure of the Ectoine utilization protein EutD (DoeA) from Halomonas elongata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	157.073, 157.073, 124.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.390 - 2.400
Rwork	0.184
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6twj
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.484
High resolution limit [Å]	2.399	2.399
Rmerge	0.190
Rpim	0.051
Number of reflections	60665	5767
<I/σ(I)>	12.02
Completeness [%]	99.1
Redundancy	14.7
CC(1/2)	0.998	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M trisodium citrate, 20 % (w/v) PEG 3350