6YNF

GAPDH purified from the supernatant of HEK293F cells: crystal form 3 of 4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97622
Spacegroup name	P 1 21 1
Unit cell lengths	87.136, 111.429, 135.943
Unit cell angles	90.00, 96.02, 90.00

Refinement procedure

Resolution	135.180 - 2.394
R-factor	0.1808
Rwork	0.179
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ynd
RMSD bond length	0.009
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3 (19-MAR-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.180	2.740
High resolution limit [Å]	2.390	2.390
Rmerge	0.270	1.280
Rmeas	0.290	1.380
Number of reflections	59243	2962
<I/σ(I)>	6.5	1.5
Completeness [%]	58.2	8.7
Redundancy	7.1	7
CC(1/2)	0.986	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	Condition F4 of MORPHEUS screen. 0.12M monosaccharides solution, 0.1M MORPHEUS Buffer System 3, 30% v/v MORPHEUS Precipitant Mix 1