EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.28 |
Number of Particle | 15165 |
Software | PHENIX,cryoSPARC,EPU,CTFFIND,Gctf,UCSF Chimera |
Symmetry | D5 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |