6YMU
Imidazole Glycerol Phosphate Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-18 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.417, 85.901, 128.745 |
| Unit cell angles | 90.00, 108.24, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.110 |
| Rwork | 0.210 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.057 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 1.500 | |
| Number of reflections | 95686 | 9472 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.990 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | PEG |
