6YML
Crystal structure of the SAM-SAH riboswitch with decarboxylated SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9188 |
Spacegroup name | P 3 1 2 |
Unit cell lengths | 86.218, 86.218, 75.395 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.050 - 2.170 |
R-factor | 0.1998 |
Rwork | 0.198 |
R-free | 0.23320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6yl5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.488 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.400 | 2.200 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.095 | 1.500 |
Rpim | 0.033 | 1.000 |
Number of reflections | 17121 | 867 |
<I/σ(I)> | 9.4 | 0.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 | |
CC(1/2) | 0.997 | 0.355 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.01 M Magnesium Sulfate, 0.05 M Sodium Cacodylate pH 6.0, 1.8 M Lithium Sulfate monohydrate |