6YMJ
Crystal structure of the SAM-SAH riboswitch with adenosine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9119 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.599, 147.570, 75.013 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 37.340 - 2.040 |
| R-factor | 0.1993 |
| Rwork | 0.198 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yl5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.479 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.990 | 2.080 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.054 | 1.300 |
| Rpim | 0.035 | 0.860 |
| Number of reflections | 90426 | 2973 |
| <I/σ(I)> | 11 | 0.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.997 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.01 M Magnesium Sulfate, 0.05 M Sodium Cacodylate pH 6.0, 1.8 M Lithium Sulfate monohydrate |
