6YMC
26-mer stem-loop RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.270, 52.430, 107.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.1784 |
| Rwork | 0.175 |
| R-free | 0.24090 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.278 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 9048 | 750 |
| <I/σ(I)> | 13.1 | 3.49 |
| Completeness [%] | 99.9 | 96 |
| Redundancy | 5.9 | 5.7 |
| CC(1/2) | 0.987 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 100 mM NaCl, 15 mM BaCl2, 40 mM Na Cacodylate 7.0 44 % MPD, 12 mM spermine tetrahydrochloride |
